2-dimensional paper chromatography

2-dimensional paper chromatography is a paper chromatographic technique in which a chromatogram is first developed using a particular solvent (either through ascending or descending paper chromatography) and then rotated by 90 degrees for a second run with another solvent.

2-dimensional paper chromatography allows components that are not separated by the first solvent (due to their insolubility in that solvent) to be separated by the second solvent, thereby producing a chromatogram with better resolution than a 1-dimensional paper chromatogram.

 

Question

With reference to the above diagram, what is the minimum number of chemical components in mixture X?

Answer

5

 

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